The 5-Q705 modification incorporates a fluorescent Quasar® 705 dye at the 5’ terminus of an oligonucleotide. 5’ Quasar 705 modified oligonucleotides can be used in a wide array of applications including dual-labeled fluorogenic probes for real-time PCR. Quasar 705 fluorophore is an indocarbocyanine dye which fluoresces in the deep red region of the visible spectrum and can be effectively quenched by either BHQ®-2 or BHQ-3 dyes.
Properties:
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Chemical Name:
Quasar® 705
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Formula:
C45H53N3O6P
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Molecular Weight:
762.89
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Absorption Maximum (Lambda Max):
690
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Extinction Coefficient at Lambda max:
206000
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Extinction Coefficient at 260 nm:
15600
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Fluorescence Maximum:
705
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
746.89