Custom NGS-grade oligos designed to improve library-prep success and minimize index crosstalk in multiplexed sequencing workflows.
Key benefits- High quality at great value: Oligos with high purity and reproducibility without the added cost of HPLC purification.
- Low cross-contamination: Consistently low oligo cross-contamination to reduce barcode crosstalk and improve data integrity in multiplexed NGS experiments.
- Flexible formulation options: Choose from single, paired, or annealed oligos to fit seamlessly into your workflow.
- Dedicated support for your larger projects: Work with our collaborative team to develop and deliver high-volume or commercial products tailored to your exact specifications.
Our NxGrade™ custom oligonucleotides production line is purpose‑built for NGS indexing primers and adapters.
At LGC Biosearch Technologies, we operate a dedicated production line with an optimized workflow specifically engineered to deliver oligos with high purity, excellent reproducibility, and maximal cross‑contamination mitigation. NxGrade oligo cross‑contamination is monitored through routine NGS‑based testing, as well as environmental and hardware monitoring using qPCR.
| Cross-contamination levels* |
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| <0.05% |
Low oligo cross-contamination in NGS indexing primers and adapters is essential for reliable sequencing results because even trace amounts of the wrong index can cause barcode misassignment, where reads are assigned to the wrong sample. Cross-contaminated or low-purity oligos, containing truncated sequences, can contribute to adapter crosstalk or index hopping. These issues can degrade data integrity, compromise downstream analysis, cause sample misidentification, waste sequencing capacity, delay projects, and increase overall costs
Traditional HPLC purification methods reliably generate high‑purity, full‑length oligos, but they can also introduce cross‑contamination from previously purified sequences.
Our reverse‑phase cartridge (RPC) purification method offers a high‑value alternative, producing oligos with high purity while minimizing cross‑contamination. RPC purification effectively removes truncated species and other impurities, without the contamination risks commonly associated with HPLC.
Specifications
| Sequence length range | 10–80 bases | ||||
| Purification type | Reverse-phase cartridge (RPC) | ||||
| Standard delivered amounts | Small: 5 nmol yield per oligo Medium: 20 nmol yield per oligo Large: 50 nmol yield per oligo |
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| Modifications |
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| Delivery format | 1 or 2 oligos per well, annealing optional Dry or in solution |
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| Vessels | Deep well 96‑well plate Racked Matrix™ tubes |
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| Cross-contamination level | <0.05%* | ||||
| Quality control | 100% mass spectrometry Analytical UPLC |
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| Quality management system | ISO 9001 |
*Typical cross-contamination levels are determined by routine NGS monitoring.
Flexible Formulation Options
Flexible custom formulation options for high-volume projects
Our dedicated, collaborative team works closely with you to customize formulation, delivery format, and labeling to meet the exact needs of your project.
Our capabilities include:- Bulk oligo production in plate format: quantities up to 100s of nmol per sequence, with a wide range of available modifications.
- Versatile delivery formats: 96-well or 384-well PCR plates, deep-well plates, or Matrix tubes.
- Ready-to-use plate options: stamped, single-use plates with heat seals for streamlined workflows.
- Custom labeling: tailored labeling to support seamless integration into your tracking and LIMS systems.
- Controlled manufacturing environment: formulations produced in an ISO 13485-certified, non-oligo synthesis facility.
- Collaborative QC development: partner with us to design and validate in-process and final QC methods, including QC plates and outgoing NGS QC.
Seeking a partner for your next project?
We are ready to assist.
Let us know what you are trying to achieve and our expert team will be in contact within a business day to talk through your needs.