Education

ValuPanel PCR assays for pathogen detection

Fluorophores and quenchers

As inventors of the Black Hole Quencher™ (BHQ™) dyes, we design our dyes with the goal of providing solutions for you, the scientist. Our vibrant fluorophores and versatile quenchers are valuable tools for many technologies including: real-time qPCR, multiplex qPCR, SNP genotyping, and FRET assays. See below to learn more about our dyes and the technologies they support.

DNA Synthesis

In addition to being a custom oligonucleotide manufacturer, LGC Biosearch Technologies is a major supplier of DNA synthesis reagents to oligo synthesis companies around the world.

Multiplexing qPCR

Multiplexed qPCR combines several PCR assays together into one but resolves the reactions using reporter dyes with distinct spectra. Only Biosearch Technologies offers fluorophores and quenchers that span the spectrum to maximise your multiplexing for 2-plex, 3-plex, 4-plex, and 5-plex qPCR.

Genotyping - qPCR

Real-time PCR methods using fluorescent probes are ideal for end-point genotyping in low to medium throughput disease-association studies, or marker selection in industrial applications. Review the probe types we offer for this application, and learn how to design assays for genotyping.

Gene expression - qPCR

Learn how LGC, Biosearch Technologies can aide your gene expression application.

Real-time PCR Probe Animation Video

Watch this video to learn about different real-time PCR probe and primer formats: Dual-labeled BHQ probes (5' Nuclease), Molecular Beacons, and Scorpions Primers.

Quenching mechanisms in oligonucleotide probes

The efficiency of fluorescence quenching upon excitation is very distance dependent – if the reporter fluorophore and quencher are far apart, there is fluorescence; if the reporter and quencher are close together in space, fluorescence is suppressed. Continue reading to learn more about static and FRET quenching.