The I-CR610 modification adds a fluorescent CAL Fluor® Red 610 dye internally or to the 5’ end of an oligonucleotide. CAL Fluor Red 610 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. The fluorophore is conjugated to a modified thymidine which may participate in hybridization. CAL Fluor Red 610 fluorophore is a xanthene dye that fluoresces in the red region of the visible spectrum and can be effectively quenched by BHQ®-2 dye. It is used as a replacement for the Texas Red® and ROX dyes.
Properties:
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Chemical Name:
T(CAL Fluor® Red 610)
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Formula:
C54H62N7O13P
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Molecular Weight:
1048.1
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Absorption Maximum (Lambda Max):
590
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Extinction Coefficient at Lambda max:
107000
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Extinction Coefficient at 260 nm:
70700
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Fluorescence Maximum:
610
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
1033.09
