The 5-CO modification adds a fluorescent CAL Fluor® Orange 560 dye to the 5’ end of an oligonucleotide. CAL Fluor Orange 560 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. CAL Fluor Orange 560 fluorophore is a xanthene dye that fluoresces in the orange region of the visible spectrum and can be effectively quenched by BHQ®-1 dye. It is used as a replacement for the VIC, HEX and JOE dyes.
Properties:
-
Chemical Name:
CAL Fluor® Orange 560
-
Formula:
C31H34N3O6P
-
Molecular Weight:
575.59
-
Absorption Maximum (Lambda Max):
538
-
Extinction Coefficient at Lambda max:
81000
-
Extinction Coefficient at 260 nm:
15000
-
Fluorescence Maximum:
559
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
560.6
