The 5-5TAM modification incorporates a fluorescent tetramethylrhodamine (TAMRA) moiety at the 5' terminus of an oligonucleotide. 5' TAMRA modified oligonucleotides can be used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. TAMRA is a rhodamine dye and this product is prepared using the 5-carboxy isomer. TAMRA fluoresces in yellow-orange region of the visible spectrum and can be effectively quenched by BHQ®-2 dye.
Properties:
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Chemical Name:
5-TAMRA
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Formula:
C37H47N3O8P
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Molecular Weight:
692.76
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Absorption Maximum (Lambda Max):
557
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Extinction Coefficient at Lambda max:
94400
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Extinction Coefficient at 260 nm:
31600
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Fluorescence Maximum:
583
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
675.76
