The 5MedC modification incorporates a 5-Methyl deoxyCytidine internally or at the 5' terminus of an oligonucleotide. When substituted for dC, 5-Methyl deoxyCytidine will increase the stability of the duplex, raising the Tm by 1.3°C per insertion. Substitution of dC with 5MedC in the CpG motif of antisense oligonucleotides can prevent or significantly reduce any unwanted pro-inflammatory immune responses in vivo. Other areas of research include the study of DNA methylation in epigenetics.
Properties:
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Formula:
C10H13N3O7P
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Molecular Weight:
318.2
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Absorption Maximum (Lambda Max):
0
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Extinction Coefficient at Lambda max:
7050
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Extinction Coefficient at 260 nm:
7050
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Fluorescence Maximum:
0
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
303.21
