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Stellaris® FISH Probes, Mouse Gapdh with Quasar® 570 Dye consists of a set of dye-labeled oligos mixed and pooled into a final delivered amount of 5 nmol, which yields approximately 200-400 hybridizations under standard conditions. Designed to detect Gapdh transcripts in specimens using fluorescence in situ hybridization (FISH).
Design Criteria: Product was designed against glyceraldehyde-3-phosphate dehydrogenase Gapdh a.k.a. Gapd (NCBI gene ID:14433) and nucleotides 241-1242 of NM_008084.3. This is an inclusive probe set designed to also detect NM_001289726. NOTE: Cross-hybridization possible to the testis-specific Gapdhs mRNA (NM_008085, NM_001290631, XM_006539545, XM_006539546), and also to RNAs from processed retrotransposed RNA pseudogenes, some of which are not annotated on reference assembly: NR_002890, NR_003623, NR_003630, NR_004447, NR_004448, NR_023357, NR_033215, NR_033630, NR_033633, NR_073535, XR_375047, XR_375048, XR_375049, XR_400290, XR_400291, XR_404002, XR_404867, XR_405643, XM_001476707, XM_006514427, XM_006529596. Note that due to potential cross-hybridization to RNA from other genes, this probe set is best used as a positive control for establishing the Stellaris RNA FISH method and not for single molecule quantification. The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species.
Properties:
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Absorption Maximum (Lambda Max):
548
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Fluorescence Maximum:
566
Product usage:
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Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month.
For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month.
Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols.
Storage and handling:
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Shipping conditions:
Ambient
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Storage conditions:
+2 to +8 °C
