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DNA encoded library (DEL) components

 

Accelerating drug discovery with better oligos

DNA encoded libraries (DEL) are a rapidly growing drug discovery platform. Synthesising a DNA encoded library requires the assembly of unique molecules linked to unique identifying DNA sequences that serve as barcodes. Subsequently, hits (candidate compounds) are identified by sequencing the DNA barcode. The use of high quality oligos is essential to the success of DEL synthesis.

High quality oligos for high quality libraries

We offer standard and custom DEL oligo components (headpieces, tags and primers) and are a trusted partner with many organizations that are synthesising DEL by split and pool, encoded self-assembly, DNA templated chemistry, and other synthesis techniques.

By understanding your needs and working collaboratively, our aim is to advance DEL technology together.

Better results

Confidence that DEL synthesis will work the first time
  • Over 35 years of experience in nucleic acid synthesis
  • 100% QC – each oligo is QC’d by LC/MS to verify quality and identity
  • Manufactured in an ISO 9001 certified facility

Reproducibility

Consistent performance in library synthesis
  • Large scale synthesis capacity allows for single batch manufacturing, removing the need to pool multiple batches
  • Proprietary in-house engineered synthesizers
  • Automated liquid handling
  • Complete control of raw material supply chain and quality

Flexibility

Get the oligos you want in the format you want
  • Multiple standard and custom synthesis scales
  • Multiple delivery formats
  • Custom modifications
  • Custom conjugation chemistries

New: DNA encoded library (DEL) starter kit

Developing your DEL platform for the first time? Our DEL starter kit makes it easy by providing all the components needed to get your pilot DEL assembly started.

The DEL starter kit contains:

  • 10 µmol AOP linker-modified headpiece
  • 10 µmol DEL primer
  • 192 tag primer pairs enabling high library complexity (100 nmol each)
  • 60,000 units (30 U/µL) of high-quality T4 DNA Ligase for efficient tag ligation.

The headpiece is a standard kit component, while the sequences of the DEL primer and DNA tags are provided by the customer. See the Technical Specs tab for more details about the kit components.

Please your order for the kit via the 'Order now' tab.

How to order (DEL) components

We offer standard and custom DEL oligo components and are a trusted partner with many organisations that are synthesizing DEL by split and pool, encoded self-assembly, DNA templated chemistry, and other synthesis techniques.

Please use the following forms to order headpiece, primers, tags and custom components. See the Technical Specs tab for product details. For order and product enquiries, please contact us at info@biosearchtech.com.

Order the new DEL starter kit here:

The DEL starter kit can be ordered by email at info@biosearchtech.com. Complete the following order forms for primers and tags and submit the forms to as per the instructions in the order form. Please state in the email your intention to purchase the DEL Starter Kit (Cat no: DEL-KIT-10).

 

Need support?

Reach out if you'd like assistance placing your order.

 

Headpiece (HP)

Available as standard and custom sequences:

  • Standard headpiece: 5'-Phos-XXXXXX[Spacer 9][Amino C7][Spacer 9]XXXXXXXX-3' 
  • AOP headpiece: 5'-Phos-XXXXXX[Spacer 9][PEG4-Amino C7][Spacer 9]XXXXXXXX-3' (See our order form for standard sequence and order information. More information about the headpiece sequence can be found at Clarke et al., Nature Chemical Biology, 2009)
  • Custom headpiece: 5'-Phos-XXXXXX[Modification][Amino C7][Modification]XXXXXXXX-3' 
  • Available in standard (14bp) or custom length
  • Custom linker modifications and primer ligated HP available
     
Label

5' phosphate
Amine-modified
Linker modification available
Amount delivered µmol to mmol
Purification AX HPLC, desalted by SEC or TFF
Format Lyophilised
QC LC/MS to verify quality and identity (>90%)
Order Standard and custom headpiece order form
standard headpiece

Standard headpiece

AOP headpiece image

AOP headpiece

Custom components

You can specify exactly what you need:

Enquire
 

Primer

Primer with custom sequence and length (generally 9-20 bp), 5’ phosphate.

Label

Available with:

5' phosphate
5' and 3' phosphate
Unmodified
Amount delivered 50 nmol to mmol amounts
Purification Desalted or HPLC available
Format Dried, single stranded or oligo pair
QC LC/MS to verify quality and identity
Order Please use the custom oligo order form and specify delivered amount under "Synthesis Scale" e.g. 1000 μmol del.

Closing primer

Primer with custom sequence and length. Upper strand is usually 10 bp and labeled with 5' phosphate. Lower strand is generally 36 bp or custom length with 5’ phosphate. It includes a degenerate region (using nucleotide code N) that varies in length and position within sequence.

Label 5' phosphate
Amount delivered 50 nmol to mmol amounts
Purification Desalted or HPLC available
Format Dried, single stranded or oligo pair
QC LC/MS to verify quality and identity
Order Please use the custom oligo order form and specify delivered amount under "Synthesis Scale" e.g. 1000 μmol del.


Tags

Paired oligos, usually between 9 and 13 bases (or custom), 5’ phos, delivered in 2D barcoded tubes as 1 mM solution in water.

Label 5' phosphate
Amount delivered 100-1,000 nmol delivered (larger scales available as custom)
Purification Desalted
Format Oligo pair, 1 mM solution in water in 2D barcode tubes, 96 tubes per rack
QC LC/MS to verify quality and identity
Order

 

DNA encoded library (DEL) starter kit (Cat. no.: DEL-KIT-10)

AOP headpiece (HP) 10 µmol of standard length (14bp) HP with a PEG-4 amine modified AOP linker.
5’-Phos XXXXXX[Spacer 9][PEG4-Amino C7][Spacer 9]XXXXXXXX-3′  
More information about the headpiece sequence can be found at (Clarke et al., Nature Chemical Biology, 2009)
DEL primer 10 µmol of 5' phosphorylated primer pair for initial HP ligation.
Primers can be 11-20 nucleotides long.
Sequences are provided by the customer.
DNA tags Up to 384 unique tag sequences, provided as 192 tags oligo pairs. 100 nmol of each tag.
Tag sequences can be 9 to 13 nucleotides long.
Sequences are provided by the customer.
NxGen™ T4 DNA Ligase 4 X 0.5 mL of high-concentration (30 U/µL) T4 DNA Ligase.
2 X 10 mL of 10X Ligase buffer.

Please download this brochure for more information,  DNA encoded library (DEL) brochure PDF.

 

Need support?

Reach out if you'd like assistance placing your order.