The 3-ROX modification adds a fluorescent carboxy-x-rhodamine (ROX) moiety to the 3’ end of an oligonucleotide. 3' ROX labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. This 3' ROX modification is conjugated to a modified cytidine which may participate in hybridization. The 3’ ROX modification also introduces a 3' phosphate which prevents extension of the oligonucleotide by DNA polymerase. ROX is a rhodamine dye that fluoresces in the red region of the visible spectrum and can be effectively quenched by BHQ®-2 dye.
Properties:
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Chemical Name:
mdC(ROX)
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Formula:
C53H63N6O14P
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Molecular Weight:
1039.07
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Absorption Maximum (Lambda Max):
586
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Extinction Coefficient at Lambda max:
91000
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Extinction Coefficient at 260 nm:
37150
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Fluorescence Maximum:
610
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
1103.06
