Fluorescein, on T; Internal Modification

The I-FAM modification incorporates a fluorescent fluorescein (FAM) moiety internally or at the 5' terminus of an oligonucleotide. FAM modified oligonucleotides can be used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. FAM is a xanthene dye and this product is prepared using the 6-carboxy fluorescein isomer. The fluorophore is conjugated to a modified thymidine which may participate in hybridization. FAM fluoresces in the green region of the visible spectrum and its fluorescence can be effectively quenched by BHQ®-1 dye.

Properties:

Chemical Name:
T(FAM)
Formula:
C39H36N4O15P
Molecular Weight:
831.71
Absorption Maximum (Lambda Max):
495
Extinction Coefficient at Lambda max:
54700
Extinction Coefficient at 260 nm:
25500
Fluorescence Maximum:
520

Product usage:

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
816.7

Storage and handling:

Fluorescein, on T; Internal Modification

Properties:

Chemical Name:

T(FAM)

Formula:

C39H36N4O15P

Molecular Weight:

831.71

Absorption Maximum (Lambda Max):

495

Extinction Coefficient at Lambda max:

54700

Extinction Coefficient at 260 nm:

25500

Fluorescence Maximum:

520

Product usage:

The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:

816.7

Storage and handling:

Trademarks
Biosearch, the Biosearch Technologies logo, Black Hole Quencher, BHQ, BHQplus, CAL Fluor, Quasar, Pulsar, Stellaris and SuperROX are registered trademarks of Biosearch Technologies, Inc.

Permitted Product Usage
All products offered for sale by Biosearch Technologies, Inc. (BTI) are sold under the condition that they be used for research purposes only and are prohibited from use in diagnostic or other commercial applications unless explicitly authorized by separate written agreement with BTI.

Black Hole Quencher® (BHQ), CAL Fluor®, Quasar® and Pulsar® dyes (referred to collectively as “BTI Dyes”) are sold to the purchaser only for internal R&D use, and are not to be used for clinical or diagnostic purposes. Neither the BTI Dyes nor the compounds synthesized with them are to be re-packaged or re-sold. Separate licenses to the BTI Dyes may be available for applications other than the aforementioned internal R&D. Please inquire via info@biosearchtech.com.

Patents
The Black Hole Quencher dye technology is protected in the United States and other countries by U.S. patents and continuations numbered 7,019,129, 7,019,129B1, 7,109,312B2, 7,582,432, 8,410,255B2 and 8,440,399B2 issued to Biosearch Technologies, Inc. The CAL Fluor technology is covered by U.S. patent number 7,344,701B2. The Quasar technology is covered by U.S. Patent numbers 7,705,150B2, 7,868, 157B2 and 8,436,153B2. The Pulsar technology is covered by U.S. Patent numbers 7,635,762B2 and 8,119,781B2.

Supercolumns are protected by U.S. Patent 6,761,855 B1, "Column for solid phase processing" issued to Biosearch Technologies, Inc. Contact licensing@biosearchtech.com for more information. Supercolumns for use on ABI 3900 or equivalent rotary cartridge style shoulder mount design are also manufactured under license from McLuen Design, U.S. Patents 8,158,085 and 8,404,196.

Stellaris products are sold under license from Rutgers University and may be used under its patent rights for Research Use Only.

Licensing
Information on licensing programs for the commercial and/or diagnostic use of these products is available by contacting licensing@biosearchtech.com.

Please visit our legal webpage on www.biosearchtech.com for full legal disclosure.