The 3-CR610 modification adds a fluorescent CAL Fluor® Red 610 dye to the 3’ end of an oligonucleotide. CAL Fluor Red 610 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. The fluorophore is conjugated to a modified thymidine which may participate in hybridization. CAL Fluor® Red 610 fluorophore is a xanthene dye that fluoresces in the red region of the visible spectrum and can be effectively quenched by BHQ®-2 dye. It is used as a replacement for the Texas Red® and ROX dyes.
Properties:
-
Chemical Name:
T(CAL Fluor® Red 610)
-
Formula:
C57H69N7O14P
-
Molecular Weight:
1107.17
-
Absorption Maximum (Lambda Max):
590
-
Extinction Coefficient at Lambda max:
99300
-
Extinction Coefficient at 260 nm:
27300
-
Fluorescence Maximum:
610
Product usage:
-
The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
1171.15
