The 5-CR590 modification adds a fluorescent CAL Fluor® Red 590 dye to the 5’ end of an oligonucleotide. CAL Fluor Red 590 labeled oligonucleotides are used in a wide array of applications, including dual-labeled fluorogenic probes for real-time PCR. CAL Fluor Red 590 fluorophore is a xanthene dye that fluoresces in the red region of the visible spectrum and can be effectively quenched by BHQ®-2 dye. It is used as a replacement for the TAMRA dye.
Properties:
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Chemical Name:
CAL Fluor® Red 590
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Formula:
C33H39N3O6P
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Molecular Weight:
604.65
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Absorption Maximum (Lambda Max):
569
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Extinction Coefficient at Lambda max:
79000
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Extinction Coefficient at 260 nm:
20900
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Fluorescence Maximum:
591
Product usage:
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The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:
588.65
